Composite

Part:BBa_K2922034:Design

Designed by: Yunyue Shi   Group: iGEM19_XMU-China   (2019-10-13)


The colicin-N operon under T7 promoter control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
    Illegal AgeI site found at 1602
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each CDS are added an RBS (BBa_B0034) behind,and terminator(BBa_B0010) is added.


Source

This part constructs from BBa_K525998,BBa_K2922027,BBa_2922028,BBa_k2922029 by standard assembly..

References